0000003517 00000 n Aging is a natural, multifactorial and multiorganic phenomenon wherein there are gradual physiological and pathological changes over time. Aldehyde dehydrogenase-2 (ALDH2) is the main enzyme responsible for acetaldehyde oxidation in ethanol metabolism and also provides protection against oxidative stress. Key words: DMBA, mammary carcinogenesis, Charles River Sprague Dawley rats 1. Conversely, the effects of aging in Col6a1−/− gastrocnemius were similar but delayed becoming apparent at 12 months of age. Frequently Asked Questions 1. Its main advantage is its calmness and ease of handling. Cancer Chemotherapy and Pharmacology 2017, 80 (2) , 317-332. Incidence of neoplastic lesions in untreated Sprague-Dawley rats (1340 males and 1329 females) used as controls in 17 carcinogenicity studies are tabulated and evaluated. SAS Sprague Dawley Rat Details. injection of pentylenetetrazol (PTZ, 70–80 mg kg −1); some pups of each litter were used as controls. Left ventricular developed pressure (LVDP) was decreased in all age groups, while heart rate (HR) was decreased in 3 and 12 months groups. Wistar rats are more active than others like Sprague Dawley rats. These results support the hypothesis of successful aging in this rat model. These patients (52 men and 11 women, with age range 49-72 years) were randomized into two groups: untreated group (n = 30) and α-LA group (n = 33). 2-AG signaling depends on the type of neurons involved in the process (4, 13–16). The Sprague Dawley rat and Long–Evans rat were developed from Wistar rats. Comparison of NTP Historical Control Tumor Incidence Rates in Female Harlan Sprague Dawley and Fischer 344/N Rats GREGG E. DINSE 1,SHYAMAL D. PEDDADA 1,SHAWN F. HARRIS 2, AND SUSAN A. ELMORE 3 1Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA 2SRA International, Inc., 2605 Meridian Parkway, Durham, NC, USA In a rat model, autologous ASC subcutaneous transplantation in post-burn scars elicited anti-neuroinflammation effects locally and in the spinal cord that might be related to the relief of post-burn neuropathic pain and attenuated cell apoptosis. Why are you such a great model? Proteins significantly altered (ANOVA and Tukey, p<0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. Conclusion: These results reinforce the possibility that OPA1 might act as an inhibitor of mitochondrial fission . We performed an extensive proteomic profiling in two skeletal muscles (diaphragm and gastrocnemius) of wild-type and collagen VI null (Col6a1−/−) mice at different ages, from 6- (adult) to 12- (aged) month-old to 24 (old) month-old. Despite a lack of left ventricular hypertrophy, MyBP-C(+/-) hearts exhibited elevated end-diastolic pressure and decreased dP/dtmax, which was normalized following dobutamine infusion. In 1991, 8 colonies were selected to form the IGS Foundation Colony using the Charles River International Genetic Standard System. When compared to wild-type animals Col6a1−/− mice displayed a general metabolic rewiring which was particularly prominent the diaphragm at 6 months of age. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. Two groups of Sprague Dawley and 2 groups of Lister-Hooded rats were controls for the gavage and diet administration. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley … Try out the SAS Sprague Dawley rat with our animal model evaluation program→ Despite these changes, bioenergetics of these cells appeared to operate normally. Frequently Asked Questions 1. – Uchino E., Tsuzuki, T. & Inoue, K. The effects of age and sex on seven elements in Sprague-Dawley rat organs. 0000004074 00000 n 37 Full PDFs related to this paper. The objective of this experiment was to determine whether changes in liver cholesterol content, plasma lipids and lipoprotein composition result from dietary manganese (Mn) deficiency in the Sprague-Dawley (SD) and the Wistar (W) rat. Thus, ASC transplantation post-burn scars shows the potential promising clinical benefits. 0000007736 00000 n Relative to the, The aim of the present study was to investigate potential alterations in the articular cartilage in a rat model of monosodium iodoacetate (MIA)‑induced osteoarthritis (OA) with or without treatment with moderate treadmill exercise. One such mechanism is insulin/insulin-like growth factor (IGF)-1 signaling. I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. However, an in vivo phototoxicity test method to evaluate this has not been … H�\�͎�@��. Its main advantage is its calmness and ease of handling. strain of Charles River Sprague Dawley rats has prolactin-induced pituitary stimulation, and therefore, the window of susceptibility for mammary tumorigenesis is absent. Toxicologic Pathology, 11 (2) (1983). At day 1 after spinal cord injury, blood-spinal cord barrier was disrupted and activation of ER stress and autophagy were involved in the rat model of trauma. Protein kinase A treatment abolished basal differences in rates of cross-bridge recruitment between MyBP-C(+/-) and wild-type myocardium. We randomized 24 rats into 4 groups as followings: Group A and B, received saline injections and autologous transplantation of ASCs 4 weeks after sham burn, respectively; Group C and D, received saline injections and autologous transplantation 4 weeks after burn injuries. In summation, the group B animals were exposed to HFHC diet supplemented with 15% fructose for 20 weeks and designated as the prediabetic group (PD) while … In conclusion, these unique proteins, the serpin family, now have demonstrated therapeutic potential for a wide variety of inflammatory diseases in both animal and human studies and represent a new approach for drug development. In contrast to light chain 3B (LC3B), the protein expression levels of BECLIN1 and sequestosome 1 (SQSTM1) were increased in the OA group. To characterize the molecular basis of physiological cardiac aging, the proteomic profiles of Sprague Dawley rat hearts of 6, 22 and 30 months were analysed by DIGE and immunoblotting. Research Triangle Park, North Carolina, USA. 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. Burn scar pain is considered as neuropathic pain. The typical life span is 2.5–3.5 years. TBP is detected in environmental matrices and biota, including human breast milk, placenta, and serum. Lower panel: Representative immunoblot images of Dynamin Like Protein-1 (Dlp1), Mitochondrial fission 1 protein (Fis1), Mitofusin-2 (Mfn2), Dynamin-like 120kDa protein (OPA1), Cyclophilin D (CypD), and NAD-dependent protein deacetylase sirtuin-3 (Sirt3) in cardiac muscles of young (white bars), old adult (gray bars) and senescent (black bars) rats. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. U.S. Department of Health and Human Services . This appears to be due to a decreased mitophagy, induced by hyperfused, elongated mitochondria. The percentage of female rats with tumors was almost double that of males, which difference was accounted for chiefly … Early murine cholestatic liver injury is via autophagy pathway. Sepsis in Sprague-Dawley Rats. The analytes were stable on the bench‐top (8 h), after three freeze–thaw cycles, in the autosampler (8 h) and as a dry extract (−80°C for 48 h). The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. in male and female F344 and Crl:CD (Sprague-Dawley [SD]) rats are rare. The wild-type Sprague Dawley rat thymus was comprised mostly of CD4/CD8 double positive cells whereas in the SRG rat, the viable cells recovered were CD4-/CD8- (Fig 1A–1C). 0000022602 00000 n B) Immunoblotting of selected proteins (identified by proteomic analysis) in young, old adult and senescent rat cardiac muscles. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. Data were normalized against the total amount of loaded proteins stained with Sypro Ruby. In order to carry out comparative studies between animal models and humans, a precise phenotypic characterization should be performed, accounting for both muscle development and metabolism (Capitanio et al., 2005(Capitanio et al., , 2009, ... Ectopic secretion of lysosomal proteases can, in fact, degradate extracellular matrix, contributing to atherosclerosis, arthritis, aneurysms formation, and other diseases . Chemotherapy-induced gastrointestinal toxicity is associated with changes in serum and urine metabolome and fecal microbiota in male Sprague–Dawley rats. Three of 105 untreated control animals developed carcinomas of the sebaceous gland (Zymbal) of the external auditory canal at an average age of 115 weeks. Reproductive Toxicology Skinned myocardium isolated from MyBP-C(+/-) hearts displayed decreased cross-bridge stiffness at half-maximal Ca(2+)-activations, increased steady-state force generation, and accelerated rates of cross-bridge recruitment at low Ca(2+)-activations (<15% and <25% of maximum, respectively). 0000001221 00000 n In most developed countries, cardiovascular diseases are among the top causes of death and their development has been shown closely related to aging. Hematological studies of Sprague Dawley rats with dextrocardia. The results revealed that IL‑1β in the serum and IALF decreased in the OAE group, whereas IL‑4 increased, and histological evaluation indicated that the OAE group had a clear treatment response. In rat hearts, 19 proteins of cellular metabolism were identified: Aldh2, aldehyde dehydrogenase (4 proteoforms); Eno3, beta-enolase (2 proteoforms); Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Ldhb, L-lactate dehydrogenase B chain; Pgam2, phosphoglycerate mutase 2; Idh3a, isocitrate dehydrogenase [NAD] subunit alpha; Coq9, ubiquinone biosynthesis protein CoQ9; Cox5b, cytochrome c oxidase subunit 5B; Etfa, electron transfer flavoprotein subunit alpha; Etfb, electron transfer flavoprotein subunit beta; Ndufa10, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 (2 proteoforms); Ndufv1, NADH dehydrogenase [ubiquinone] flavoprotein 1; Ckb, creatine kinase B-type; Acadl, long-chain specific acyl-CoA dehydrogenase; Acsf2, acyl-CoA synthetase family member 2; Apoa1, apolipoprotein A-I; Ech1, delta(3,5)-delta(2,4)-dienoyl-CoA isomerase; Hadh, mitochondrial hydroxyacyl-coenzyme A dehydrogenase; Hadha, trifunctional enzyme subunit alpha. Sprague-Dawley rats, from prenatal life to spontaneous death: plan of the experiment (Experiment BT 1CEMRF). Join ResearchGate to find the people and research you need to help your work. Perturbations in mechanical and electrical activity in MyBP-C(+/-) mice could increase their susceptibility to cardiac dysfunction and arrhythmia. Furthermore, electrocardiogram recordings in conscious MyBP-C(+/-) mice revealed prolonged QRS and QT intervals, which are known risk factors for cardiac arrhythmia. National Toxicology Program . In rat hearts, 11 contractile and structural proteins were identified: Actc1, actin alpha cardiac muscle 1; Csrp3, cysteine and glycine-rich protein 3; Des, desmin (2 proteoforms); Mybpc3, myosin-binding protein C (2 proteoforms); Myh6, myosin-6 (8 proteoforms); Myh7, myosin-7 (5 proteoforms); Myl4, myosin light chain 4; Myl7, myosin regulatory light chain 2 atrial isoform; Tnni3, troponin I; Tubb4b, tubulin beta-4B chain; Vim, vimentin. 2. DOI: 10.1007/s00280-017-3364-z. Literature data show beneficial effects of exercise on the impairment of mitochondrial biogenesis and dynamics, and on the decrease in the mitophagic capacity associated to aging. This suggests that Cucumis sativus extract possesses antidiabetic effect on alloxaninduced diabetic - Sprague-Dawley rat and therefore can be used as an alternative therapy in treating diabetes mellitus. 0000006365 00000 n The content of methionine sulfoxides also increased for 15 of the 16 methionine residues, with Met(44), Met(47), and Met(355) having the highest sulfoxide contents. and prepared for stereotaxic infusion of drug (0–10 nmol ferrous citrate and/or 0–16.8 nmol GSNO in 1 μl sterilized Ringer's solution or 0–33.6 nmol peroxynitrite in 1.05 μl 0.3 M NaOH solution) into right or left substantia nigra compacta area (i.n. Reproductive Toxicology This lack of functional consequences was likely due to a compensatory phenomenon at the level of mitochondria, which displayed a maintained supercomplexes organization and an increased mass. However, results about the role of Fis1 protein in the aging process are not conclusive [30,44,45,48,58,59,, ... A possible explanation may be that Bnip3 induction compensates for the loss of mitochondrial autophagy and minimizes mitochondrial damage . The Wistar rat is currently one of the most popular rats used for laboratory research. The studies discussed range from thrombosis and hemorrhage to vascular disease and transplant rejection and finally to sepsis and clinical studies in humans. The aim was to detect the anti-neuroinflammation effect of ASC transplantation and, Increased levels of reactive oxygen species (ROS) cause oxidative stress and are believed to play a key role in the development of age-related diseases and mammalian aging in general by oxidizing proteins, lipids, and DNA. Mitochondria have been considered for long time as important determinants of cell aging because of their role in the production of reactive oxygen species. Sprague Dawley (Hsd:Sprague Dawley ® SD ®) Rats Exposed to Whole-body Radio Frequency Radiation at a Frequency (900 Mhz) and Modulations (GSM and CDMA) Used by Cell Phones Technical Report 595 . To SASCO from ARS/Sprague Dawley in 1979. Inhibition of ER stress by treating with 4-phenylbutyric acid decreased blood-spinal cord barrier permeability, prevented the loss of tight junction (TJ) proteins and reduced autophagy activation after spinal cord injury. Alterations in articular cartilage and synovium were also evaluated by histology, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. Asian Journal of Biomedical and Pharmaceutical Sciences; 04 (37); 2014,33-37. Proteoform: A single term describing protein complexity, Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria, Development of a strain spontaneously hypertensive rats, Specific protein changes contribute to the differential muscle mass loss during ageing, The Serpin Solution; Targeting Thrombotic and Thrombolytic Serine Proteases in Inflammation, Alpha-Lipoic Acid Ameliorates Oxidative Stress by Increasing Aldehyde Dehydrogenase-2 Activity in Patients with Acute Coronary Syndrome, Hexosamine Biosynthetic Pathway as target for cancer therapy, Autologous Adipose-Derived Stem Cells Reduce Burn-Induced Neuropathic Pain in a Rat Model. Public Health Service . MKK7 mRNA level was increased in 24 months group and p-MKK7 levels were increased in 12 months group following Sunitinib treatment. ��%�| 9�7;�E10�� ,xr[�R���K�L�ʡ����Dc ������2�4#�0 �� endstream endobj 15 0 obj <>>> endobj 16 0 obj <>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 595.276 841.89]/Type/Page>> endobj 17 0 obj <> endobj 18 0 obj <> endobj 19 0 obj <> endobj 20 0 obj <> endobj 21 0 obj <> endobj 22 0 obj <> endobj 23 0 obj <> endobj 24 0 obj <>stream gestational day (GD) 6 of their mothers. The rats are maintained as an outbred closed colony. evaluated upon completing the treadmill exercise program (speed, 18 m/min; 30 min/day; 5 days/week for 4 weeks). A spontaneous tumor incidence of 45% was noted in 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. 0000001122 00000 n MKK7 mRNA and p-MKK7 levels were decreased in the 3 months group after Sunitinib treatment. Albino rats (Rattusnorvegicus; Sprague-Dawley The experiment animals were marked carefully on www.pharmascholars.com 147 Rakib Hasan, et al. The pure computationally inferred model presented in the paper is therefore a good candidate to represent the relationship between key genes and miRs involved in cardiac senescence processes, and represents a reliable selection of genes and miRs for further studies, in order to elucidate and better detail their involvement in cardiac aging. 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